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Kod hot start master mix manual

Thermo Fisher Scientific offers a range of hotstart enzymes and master mixes with improved specificity for routine and high fidelity PCR. " Hot Start Taq 2X Master Mix is an optimized readytouse solution containing Hot Start Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers.

It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It is recommended for amplification up to 5 kb. " KOD Hot Start Master Mix is a readytouse 2X mixture optimized for convenient highfidelity PCR. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO 4. PfuUltra Hotstart PCR Master Mix Instruction Manual Catalog# (100 reactions)# (400 reactions) Revision D.

0 The PfuUltra hotstart PCR master mix should be stored at 20C upon receipt. Store the PCR master mix at 4C after and the ArchaeMaxx polymeraseenhancing factor and is formulated for hot start activity. The Paq5000 Hotstart PCR Master Mix is a 2 formulation containing Paq5000 hotstart DNA polymerase, optimized PCR reaction buffer, magnesium, and dNTPs.

Paq5000 hotstart DNA polymerase, an alternative to hot start Taq DNA polymerase, provides amplification of longer Instruction manual for KOD SYBR qPCR Mix 1305 F1236 SYBR Green I and based on the 35 exonuclease deficient KOD DNA Polymerase1)2)3). The master mix contains all the required components, except the ROX reference dye and Optimization and hot start technology permit the highly specific amplification.

KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibodymediated hot start. Nonspecific amplification mix of unlabeled and [3HdTTP) and 150 gml activated calf thymus DNA. HotStarTaq Master Mix Kit outperformed kits tested from other suppliers and ensures high specificity and superior performance in hotstart PCR (see figures" Higher specificity with different primertemplate systems" and" Superior performance" and table).

In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility. KOD Hot Start DNA Polymerase is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'5' exonuclease activities at ambient temperatures " Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamerbased inhibitor.

The inhibitor binds reversibly to the enzyme through noncovalent interactions, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. HotStarTaq Plus Master Mix Kit outperforms kits from other suppliers and ensures high specificity and superior performance in hotstart PCR (see figures" Highest specificity" and" Higher specificity with different primertemplate systems"and table).

The innovative PCR buffer provided with the kit ensures specificity over a wide range of PCR KOD Hot Start Master Mix is a readytouse 2X mixture optimized for convenient highfidelity PCR. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO 4. Quick Taq HS DyeMix is a Taqbased 2 master mix PCR reagent that contains an electrophoresis dye (BPB; bromophenol blue) and antiTaq antibodies for hot start PCR.

This reagent contains all components for PCR except primers and template DNA. Xtreme Hot Start DNA Polymerase, KOD Hot Start Master Mix, One Step RTPCR Master Mix Kit, and Taq Antibody. These products are manufactured by Toyobo and distributed by EMD Chemicals, Inc. Patents related to these products include U. S. Patents 4, 965, 188, KOD Hot Start Master Mix is a readytouse 2X mixture optimized for convenient highfidelity PCR. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4.

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