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Qiagen rneasy plus manual

RNeasy Plus Universal Kits integrate phenolguanidinebased sample lysis and silicamembrane purification of total RNA. QIAzol Lysis Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of all kinds of tissues and to inhibit RNases.

Manual (centrifugation) Purification of total RNA, miRNA, poly A mRNA, DNA or protein. Total RNA. Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit? Contact QIAGEN: For purification of total RNA from animal cells and easytolyse animal tissues using gDNA Eliminator columns Why are researchers falling in love with RNeasy?

RNeasy Plus Kits give you high yields of highintegrity RNA in minutes for any downstream application, without the need for DNase. Discover the right QIAGEN RNeasy Kit for your workflow today! Sample to Insight. Investor Relations; Press& Media; RNeasy Micro Handbook 5 All due care and attention should be exercised in the handling of the products.

We recommend all users of QIAGEN products to adhere to the NIH guidelines that have been developed for recombinant DNA experiments, or to other applicable guidelines. RNeasy Mini Handbook (EN) Print Bookmark Qiagen rneasy plus manual RNeasy Mini Kit For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit For purification of RNeasy Plus Universal Kits integrate phenolguanidinebased sample lysis and silicamembrane purification of total RNA.

QIAzol Lysis Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of all kinds of tissues and to inhibit RNases. The RNeasy Fibrous Tissue Mini Kit includes proteinase K for removing abundant protein in fiberrich tissue samples, and RNeasy spin columns for purifying up to 100 g of highquality RNA.

RNeasy Fibrous Tissue Mini Handbook. For purification of total RNA from heart, skeletal muscle, aorta and other fiberrich tissues RNeasy Plus The RNeasy Plus Kits are next generation QIAGEN RNA extraction kits, combining highquality RNA purification with effective oncolumn gDNA removal. October 2005 WWW. QIAGEN. COM RNeasy Plus Mini Handbook For purification of total RNA from animal cells and easytolyse animal The RNeasy Plus Mini Kit integrates fast, convenient purification of up to 100 g RNA with effective elimination of genomic DNA.

Cell or tissue lysates are spun through gDNA Eliminator spin columns to remove genomic DNA. WWW. QIAGEN. COM QIAvac 24 Plus Handbook and RNeasy Spin Columns on the QIAvac 24 Plus Procedure 1.

Insert up to 24 spin columns into the luer slots of the QIAvac 24 Plus. Close unused luer slots with luer plugs. 2. Connect the upper threaded hole of the QIAvac 24 Plus manifold to RNeasy MiniHandbook RNeasy Mini Kit For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup visit www.

qiagen. com. Contents KitContents 4 Storage 5 IntendedUse 5 SafetyInformation 6 QualityControl 6 and wash steps are performed on a QIAvac 24 or QIAvac 24 Plus manifold, and the For purification of total RNA using gDNA Eliminator columns from small samples, including animal and human cells (5 x 105) animal and human tissues (5 mg) The RNeasy Micro Kit is designed for purification of up to 45 g RNA from small cell and tissue samples.

QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro Contact QIAGEN: