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Fugene hd transfection manually

FuGENE HD Transfection Reagent is a multicomponent reagent that forms a complex with DNA, then transports the complex into animal or insect cells. It is suitable for use in media with or without serum, and for transient or stable transfection, as well as cotransfections of multiple DNA plasmids. The FuGENE HD Transfection Reagent is a nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity.

The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagentDNA complex.

FuGENE HD Transfection Reagent is a multicomponent reagent that forms a complex with DNA, then transports the complex into animal or insect cells. This reagent is suitable for use in media with or without serum, and for transient or stable transfection. FuGENE HD Transfection Reagent Manual Author: Active Motif, Inc. FuGENE is a registered trademark of Fugent, LLC in the United States and other countries. FuGENE6, and FuGENEHD are trademarks of Fugent, LLC. FuGENE HD Transfection Reagent offers unrivaled transfection performance with minimal impact on cell physiology (Jacobson et al.

[2009 Biotechniques 47, ). It is ideal for use in a variety of challenging applications, including transfection of cancer model cell lines, insect cells, stem cells, and for virus and protein production.

The FuGENE HD Protocol Database is a collection of protocols that guide the transfection process for a variety of cell lines. This database will also calculate the volumes needed for transfection protocol based on well format and the number of samples. 1. Product Description A. Description FuGENE HD Transfection Reagent is a proprietary, nonliposomal, low toxicity formulation designed for high efficiency delivery of DNA into a broad range of cell lines.

The use of FuGENE HD does not require culture medium change or serum removal during transfection experiment. Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. It may also refer to other methods and cell types, although other terms are often preferred: FuGENE 6 Transfection Reagent is a nonliposomal formulation designed to transfect plasmid DNA into a wide variety of cell lines with high efficiency and low toxicity.

FuGENE HD Transfection Reagent is a proprietary, nonliposomal, low toxicity formulation designed for high efficiency delivery of DNA into a broad range of cell lines. The use of FuGENE HD does not require culture medium change or serum removal during transfection experiment. FuGENE HD Transfection Reagent offers unrivaled transfection performance with minimal impact on cell physiology (Jacobson et al.

[2009 Biotechniques 47, ). It is ideal for use in a variety of challenging applications, including transfection of cancer model cell lines, insect cells, stem cells, and for virus and protein production. The FuGENE HD Transfection Reagent is a nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagentDNA complex.

FuGENE is a registered trademark of Fugent, LLC in the United States and other countries. FuGENE6, and FuGENEHD are trademarks of Fugent, LLC. FuGENE HD Transfection Reagent can be used in transfection protocols in the presence of serum (up to 100), allowing transfection of cell types that require continuous exposure to serum, such as primary cell cultures. FuGENE 6 Transfection Reagent is stabilized for extended storage at 2 to 8C through the expiration date printed on the label (two years from the date of manufacture) when very tightly closed.